Modified semiautomated method for free fatty acids in serum.
نویسندگان
چکیده
منابع مشابه
Modified semiautomated method for free fatty acids in serum.
Modifications have been made to the reagent system of the semiautomated method for the determination of plasma free fatty acids by Baird et al. (1967), and results are reported of investigations into parameters affecting sensitivity and analytical range.
متن کاملA novel semiautomated method for the estimation of free fatty acid in serum or plasma.
A modification of the semiautomated assay method of Antonis (1965. J. Lipid Res. 6:307-312) for free fatty acid is presented. Free fatty acids are extracted from serum or plasma into di-n-butyl ether-2-methoxyethanol; the extract is almost free from phospholipids. The acids are analyzed in a portion of extract by a copper soap method using diphenylcarbazide. The extractant, being less dense tha...
متن کاملSemiautomated Method for the Colorimetric Determination of Plasma Free Fatty Acids.
A semiautomated procedure is described for the estimation of plasma free fatty acids. The method requires the preliminary preparation of a phospholipid-free plasma lipid extract which is then analyzed automatically by a colorimetric procedure based on the solubility of copper soaps in chloroform, with subsequent complexing of the copper with diethyldithiocarbamate and measurement of the extinct...
متن کامل"a gas chromatographic method for the determination of individual free fatty acids in serum"
serum fatty acids on 30 healthy lranian women were detemined by a gas chromatographic method. oleic and stearic acids were used as standards. the mean values were myristic acid 5. 7% , palmitic acid 43% , palmitoleic acid 5.3% , stearic acid 8% , oleic acid 21. 3% and linleic acid 16.4% of total fatty acid serum contents.
متن کاملColorimetric method for free fatty acids in serum validated by comparison with gas chromatography.
We compared results by a simplified colorimetric copper-soap method for serum free fatty acids (Clin, Chem. 19: 419, 1973, modified) with those by a gas--liquid chromatographic method. The modified method requires only 100 microliters of sample, its standard curve is linear from 0.1 to 4.0 mmol/L (reference interval for adults: 0.2 to 0.8 mmol/L), and it is suitable for use with newborns. Compa...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1977
ISSN: 0021-9746
DOI: 10.1136/jcp.30.8.754